"polyA substitutes in the hybridisation buffer?"

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albajc Nov-12-01, 10:36 PM (PDT) "polyA substitutes in the hybridisation buffer?" Hi, I read somewhere that a research group has been using BSA instead of polyA and cot-1 in their hybridisation buffer and had good results. Sadly, I can't remember where I read it, so has anyone else seen this? Also, as a microbiological microarrayer, what can/should I use instead of cot-1 if BSA isn't suitable?   Alert Edit | Reply | Reply With Quote | Top

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RE: polyA substitutes in the hybrid..., Kazuo, Nov-16-01, (1)

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Kazuo Nov-16-01, 01:42 PM (PDT) 1. "RE: polyA substitutes in the hybridisation buffer?" I recall that on the web site of Telechem they recommend to use BSA as a blocking agent, but I do not know what blocking agents they use besides BSA. http://arrayit.com/Products/Hybridization/Cassettes/cassettes.html   Remove | Alert Edit | Reply | Reply With Quote | Top

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