"hybridization chambers"

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florian Aug-01-01, 02:57 AM (PDT) "hybridization chambers" I am currently trying to find out which hyb chambers are really good. Is it really necessary to pay e.g. 175$ for the TeleChem chambers, or does anybody know a machinist who does this much cheaper? And what do you think about using plastic parts in hyb chambers? When you use them quite often, won´t the plastic be distorted? Please tell me about your experiences. Thank you, Florian   Alert Edit | Reply | Reply With Quote | Top

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RE: hybridization chambers, Kazuo, Aug-01-01, (1)

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Kazuo Aug-01-01, 09:49 AM (PDT) 1. "RE: hybridization chambers" Before we use an automated hyb machine, we had used Corning's hybridization chambers for array experiments. It costs $50 per chamber and it's good enough for this purpose. Since there are no screw-heads on the upper surface, you can pile them up in a water bath or a hyb oven. http://www.corning.com/CMT/Products/Hybridization.asp In some protocol (I found it on the web site of NEN), they recommended to use a 50mL-conical tube with a wet dust-free tissue (e.g. small KimWipe with 400 uL 2XSSC)as a hyb chamber. It must be one of the most ecomomical ways. You need to pay attention to keep the array on the level. Save money!   Remove | Alert Edit | Reply | Reply With Quote | Top

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