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"aRNA labelling with aadUTP and random hexamers?"

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Terry Click to EMail TerryClick to check IP address of the poster Oct-19-01, 11:15 AM (PDT)
"aRNA labelling with aadUTP and random hexamers?"
Is the protocol for aadUTP labelling (from Rosetta Infarmatics
and Joe Derisi) suitable for labelling aRNA (amplified antisense
RNA)? The only difference would be using random hexamers
and not oligo dT---correct? Is there any reason
why there would be a problem?

(The only other protocol I saw for aRNA with random hexamers
was with direct incorporation dUTP)

Does anyone know about this???

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