Recent Publications from the Crabtree Laboratory
Rapid and reversible epigenome editing by endogenous chromatin regulators.
Braun SMG, Kirkland JG, Chory EJ, Husmann D, Calarco JP, Crabtree GR.
Nat Commun. 2017 Sep 15;8(1):560. doi: 10.1038/s41467-017-00644-y.
PMID:28916764
DNA binding drives the association of BRG1/hBRM bromodomains with nucleosomes.
Morrison EA, Sanchez JC, Ronan JL, Farrell DP, Varzavand K, Johnson JK, Gu BX, Crabtree GR, Musselman CA.
Nat Commun. 2017 Jul 14;8:16080. doi: 10.1038/ncomms16080.
PMID:28706277
Chd8 Mutation Leads to Autistic-like Behaviors and Impaired Striatal Circuits.
Platt RJ, Zhou Y, Slaymaker IM, Shetty AS, Weisbach NR, Kim JA, Sharma J, Desai M, Sood S, Kempton HR, Crabtree GR, Feng G, Zhang F.
Cell Rep. 2017 Apr 11;19(2):335-350. doi: 10.1016/j.celrep.2017.03.052.
PMID: 28402856
TOP2 synergizes with BAF chromatin remodeling for both resolution and formation of facultative heterochromatin.
Miller EL, Hargreaves DC, Kadoch C, Chang CY, Calarco JP, Hodges C, Buenrostro JD, Cui K, Greenleaf WJ, Zhao K, Crabtree GR.
Nat Struct Mol Biol. 2017 Apr;24(4):344-352. doi: 10.1038/nsmb.3384. Epub 2017 Feb 27.
PMID: 28250416
Dynamics of BAF-Polycomb complex opposition on heterochromatin in normal and oncogenic states.
Kadoch C, Williams RT, Calarco JP, Miller EL, Weber CM, Braun SM, Pulice JL, Chory EJ, Crabtree GR.
Nat Genet. 2017 Feb;49(2):213-222. doi: 10.1038/ng.3734. Epub 2016 Dec 12.
PMID:27941796
Smarca4 ATPase mutations disrupt direct eviction of PRC1 from chromatin.
Stanton BZ, Hodges C, Calarco JP, Braun SM, Ku WL, Kadoch C, Zhao K, Crabtree GR.
Nat Genet. 2017 Feb;49(2):282-288. doi: 10.1038/ng.3735. Epub 2016 Dec 12.
PMID:27941795
Murine esBAF chromatin remodeling complex subunits BAF250a and Brg1 are necessary to maintain and reprogram pluripotency-specific replication timing of select replication domains.
Takebayashi S, Lei I, Ryba T, Sasaki T, Dileep V, Battaglia D, Gao X, Fang P, Fan Y, Esteban MA, Tang J, Crabtree GR, Wang Z, Gilbert DM.
Epigenetics Chromatin. 2013 Dec 13;6(1):42. doi: 10.1186/1756-8935-6-42.
PMID: 24330833
Creating a neural specific chromatin landscape by npBAF and nBAF complexes.
Staahl BT, Crabtree GR.
Curr Opin Neurobiol. 2013 Dec;23(6):903-13. doi: 10.1016/j.conb.2013.09.003.
PMID:24090879
Reprogramming human fibroblasts to neurons by recapitulating an essential microRNA-chromatin switch.
Tang J, Yoo AS, Crabtree GR.
Curr Opin Genet Dev. 2013 Oct;23(5):591-8. doi: 10.1016/j.gde.2013.07.001.
PMID:24035011
Kinetic analysis of npBAF to nBAF switching reveals exchange of SS18 with CREST and integration with neural developmental pathways.
Staahl BT, Tang J, Wu W, Sun A, Gitler AD, Yoo AS, Crabtree GR.
J Neurosci. 2013 Jun 19;33(25):10348-61. doi: 10.1523/JNEUROSCI.1258-13.2013.
PMID: 23785148
Exome sequencing to identify de novo mutations in sporadic ALS trios.
Chesi A, Staahl BT, Jovi?i? A, Couthouis J, Fasolino M, Raphael AR, Yamazaki T, Elias L, Polak M, Kelly C, Williams KL, Fifita JA, Maragakis NJ, Nicholson GA, King OD, Reed R, Crabtree GR, Blair IP, Glass JD, Gitler AD.
Nat Neurosci. 2013 Jul;16(7):851-5. doi: 10.1038/nn.3412. Epub 2013 May 26.
PMID: 23708140
BAF complexes facilitate decatenation of DNA by topoisomerase IIα.
Dykhuizen EC, Hargreaves DC, Miller EL, Cui K, Korshunov A, Kool M, Pfister S, Cho YJ, Zhao K, Crabtree GR.
Nature. 2013 May 30;497(7451):624-7. doi: 10.1038/nature12146. Epub 2013 May 22.
PMID:23698369
Proteomic and bioinformatic analysis of mammalian SWI/SNF complexes identifies extensive roles in human malignancy.
Kadoch C, Hargreaves DC, Hodges C, Elias L, Ho L, Ranish J, Crabtree GR.
Nat Genet. 2013 Jun;45(6):592-601. doi: 10.1038/ng.2628. Epub 2013 May 5.
PMID: 23644491
From neural development to cognition: unexpected roles for chromatin.
Ronan JL, Wu W, Crabtree GR.
Nat Rev Genet. 2013 May;14(5):347-59. doi: 10.1038/nrg3413. Epub 2013 Apr 9. Review.
PMID: 23568486
Reversible disruption of mSWI/SNF (BAF) complexes by the SS18-SSX oncogenic fusion in synovial sarcoma.
Kadoch C, Crabtree GR.
Cell. 2013 Mar 28;153(1):71-85. doi: 10.1016/j.cell.2013.02.036.
PMID: 23540691
The neuron-specific chromatin regulatory subunit BAF53b is necessary for synaptic plasticity and memory.
Vogel-Ciernia A, Matheos DP, Barrett RM, Kramár EA, Azzawi S, Chen Y, Magnan CN, Zeller M, Sylvain A, Haettig J, Jia Y, Tran A, Dang R, Post RJ, Chabrier M, Babayan AH, Wu JI, Crabtree GR, Baldi P, Baram TZ, Lynch G, Wood MA.
Nat Neurosci. 2013 May;16(5):552-61. doi: 10.1038/nn.3359. Epub 2013 Mar 24.
PMID: 23525042
Rethinking our intellectual origins: response to Kalinka et al.
Crabtree GR.
Trends Genet. 2013 Mar;29(3):127-9. doi: 10.1016/j.tig.2013.01.013. Epub 2013 Feb 17. No abstract available.
PMID: 23422050
ACTL6a enforces the epidermal progenitor state by suppressing SWI/SNF-dependent induction of KLF4.
Bao X, Tang J, Lopez-Pajares V, Tao S, Qu K, Crabtree GR, Khavari PA.
Cell Stem Cell. 2013 Feb 7;12(2):193-203. doi: 10.1016/j.stem.2012.12.014.
PMID:23395444
Our fragile intellect. Part II.
Crabtree GR.
Trends Genet. 2013 Jan;29(1):3-5. doi: 10.1016/j.tig.2012.10.003. Epub 2012 Nov 12.
PMID:23153597
Our fragile intellect. Part I.
Crabtree GR.
Trends Genet. 2013 Jan;29(1):1-3. doi: 10.1016/j.tig.2012.10.002. Epub 2012 Nov 12.
PMID:23153596
The BAF53a subunit of SWI/SNF-like BAF complexes is essential for hemopoietic stem cell function.
Krasteva V, Buscarlet M, Diaz-Tellez A, Bernard MA, Crabtree GR, Lessard JA.
Blood. 2012 Dec 6;120(24):4720-32. doi: 10.1182/blood-2012-04-427047. Epub 2012 Sep 26.
PMID: 23018638
Screening for inhibitors of an essential chromatin remodeler in mouse embryonic stem cells by monitoring transcriptional regulation.
Dykhuizen EC, Carmody LC, Tolliday N, Crabtree GR, Palmer MA.
J Biomol Screen. 2012 Oct;17(9):1221-30. Epub 2012 Aug 1.
PMID: 22853929
Dynamics of inherently bounded histone modification domains.
Hodges C, Crabtree GR.
Proc Natl Acad Sci U S A. 2012 Aug 14;109(33):13296-301. doi: 10.1073/pnas.1211172109
PMID:22847427
Medulloblastoma exome sequencing uncovers subtype-specific somatic mutations.
Pugh TJ, Weeraratne SD, Archer TC, Pomeranz Krummel DA, Auclair D, Bochicchio J, Carneiro MO, Carter SL, Cibulskis K, Erlich RL, Greulich H, Lawrence MS, Lennon NJ, McKenna A, Meldrim J, Ramos AH, Ross MG, Russ C, Shefler E, Sivachenko A, Sogoloff B, Stojanov P, Tamayo P, Mesirov JP, Amani V, Teider N, Sengupta S, Francois JP, Northcott PA, Taylor MD, Yu F, Crabtree GR, Kautzman AG, Gabriel SB, Getz G, Jäger N, Jones DT, Lichter P, Pfister SM, Roberts TM, Meyerson M, Pomeroy SL, Cho YJ.
Nature. 2012 Aug 2;488(7409):106-10. doi: 10.1038/nature11329.
PMID:22820256
Neonatal β cell development in mice and humans is regulated by calcineurin/NFAT.
Goodyer WR, Gu X, Liu Y, Bottino R, Crabtree GR, Kim SK.
Dev Cell. 2012 Jul 17;23(1):21-34. doi: 10.1016/j.devcel.2012.05.014.
PMID:
22814600 Free PMC Article
Dynamics and memory of heterochromatin in living cells.
Hathaway NA, Bell O, Hodges C, Miller EL, Neel DS, Crabtree GR.
Cell. 2012 Jun 22;149(7):1447-60. doi: 10.1016/j.cell.2012.03.052. Epub 2012 Jun 14.
PMID: 22704655
Staahl BT, Tang J, Wu W, Sun A, Gitler AD, Yoo AS, Crabtree GR. Kinetic Analysis of npBAF to nBAF Switching Reveals Exchange of SS18 with CREST and Integration with Neural Developmental Pathways. J Neurosci. 2013 Jun 19;33(25):10348-61. PMID: 23785148
Chesi A, Staahl BT, Jovi?i? A, Couthouis J, Fasolino M, Raphael AR, Yamazaki T, Elias L, Polak M, Kelly C, Williams KL, Fifita JA, Maragakis NJ, Nicholson GA, King OD, Reed R, Crabtree GR, Blair IP, Glass JD, Gitler AD. Exome sequencing to identify de novo mutations in sporadic ALS trios. Nat Neurosci. 2013 Jul;16(7):851-5. PMID: 23708140
Dykhuizen E, Hargreaves D, Cho YJ, et.al. BAF (mSWI/SNF) Complexes Facilitate Decatenation of DNA by Topoisomerase IIα Nature 2013 May 30;497(7451):624-7 PMID: 23698369
Kadoch Cigall, Hargreaves Diana, Hodges Courtney, Elias Laura, Ho Lena, Ranish Jeff, Crabtree Gerald. Proteomic and Bioinformatic Analysis of mSWI/SNF (BAF) Complexes Reveals Extensive Roles in Human Malignancy, Nature Genetics 2013 May 5; 45(6): 592–601 PMCID: PMC3667980
Ronan, Jehnna, Wu, Wei, Crabtree GR. From Neural Development to Cognition: Unexpected Roles for Chromatin, Nat Rev Genet. 2013 May 14(5):347-59 PMID: 23568486
Kadoch, Cigall, Crabtree GR. Reversible Disruption of mSWI/SNF-like BAF Complexes by the SS18-SSX Oncogenic Fusion in Synovial Sarcoma Cell 2013 March 28; 153(1): 71–85 PMCID: PMC3655887
Bao X, Tang J, Lopez-Pajares V, Tao S, Qu K, Crabtree GR, Khavari PA. ACTL6a Enforces the Epidermal Progenitor State by Suppressing SWI/SNF-Dependent Induction of KLF4 Cell Stem Cell. 2013 February 7; 12(2): 193–203
Krasteva V, Buscarlet M, Diaz-Tellez A, Bernard MA, Crabtree GR, Lessard JA.. The BAF53a Subunit of SWI/SNF-like BAF Complexes is Essential for Hemopoietic Stem Cell Function. Blood 2012 Dec 6;120(24):4720-32
Hodges C and Crabtree GR. Dynamics of inherently bounded histone modification domains PNAS 2012 August 14; 109(33): 13296–13301PMCID
Pugh TJ, Weeraratne SD, Archer TC, Pomeranz Krummel DA, Auclair D, Bochicchio J, Carneiro MO, Carter SL, Cibulskis K, Erlich RL, Greulich H, Lawrence MS, Lennon NJ, McKenna A, Meldrim J, Ramos AH, Ross MG, Russ C, Shefler E, Sivachenko A, Sogoloff B, Stojanov P, Tamayo P, Mesirov JP, Amani V, Teider N, Sengupta S, Francois JP, Northcott PA, Taylor MD, Yu F, Crabtree GR, Kautzman AG, Gabriel SB, Getz G, Jäger N, Jones DT, Lichter P, Pfister SM, Roberts TM, Meyerson M, Pomeroy SL, Cho YJ. Medulloblastoma exome sequencing uncovers subtype-specific somatic mutations. Nature. 2012 August 2; 488(7409): 106–110.
Nathaniel A. Hathaway, Oliver Bell, Courtney Hodges, Erik L. Miller, Dana S. Neel, Gerald R. Crabtree. Dynamics and Memory of Heterochromatin in Living Cells Cell, 2012 Jun 22;149(7):1447-60.
Goodyer WR, Gu X, Liu Y, Bottino R, Crabtree GR, Kim SK. Neonatal β Cell Development in Mice and Humans Is Regulated by Calcineurin/NFAT. Goodyer Dev Cell. 2012 Jul 17;23(1):21-34.
Yoo AS, Sun Alfred, Crabtree GR, MicroRNA-Mediated Conversion of Human Fibroblasts to Neurons Nature 2011 Jul 13;476(7359):228-31. PMCID: PMC3155811
Ho, L, Miller Erik L., Ronan Jehnna, Ho WQ, Jothi R, Crabtree GR. esBAF Facilitates Pluripotency by Conditioning the Genome for LIF/STAT3 Signaling and by Regulating Polycomb Function Nature Cell Biology 2011 Jul 24;13(8):903-13. PMID: 21785422
Liang FS, Ho WQ, Crabtree GR. Engineering the ABA Plant Stress Pathway for Regulation of Induced Proximity. Sci Signal. 2011 Mar 15;4(164):rs2. PMCID: PMC3110149
Hargreaves DC, Crabtree GR ATP-dependent chromatin remodeling: genetics, genomics and mechanisms. Cell Res. 2011 Mar;21(3):396-420. PMID: 21358755
Lessard J, Crabtree G.R. Chromatin Regulatory Mechanisms in Pluripotency Annu Rev Cell Dev Biol. 2010 Jul 12
Ho L, and Crabtree GR Chromatin remodeling during development Nature 2010 Jan 28
Crabtree, GR and Schreiber, SL SnapShot: Calcineurin/NFAT Signaling Cell, 138, 210 2009
Yoo, Andrew S., Staahl, Brett T, Chen, Lei and Gerald R. Crabtree MicroRNA-Mediated Switching of Chromatin Remodeling Complexes in Neural Development. Nature, July 2009
Yoo AS, Crabtree GR.ATP-dependent chromatin remodeling in neural development.Curr Opin Neurobiol. 2009 May 11
Lena Ho, Raja Jothi, Jehnna L. Ronan, Kairong Cui, Keji Zhao, Gerald R. Crabtree, An Embryonic Stem Cell Chromatin Remodeling Complex, esBAF is an Essential Component of the Core Pluripotency Transcriptional Network Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5187-91.
Lena Hoa, Jehnna L. Ronanb, Jiang Wuc, Brett Staahld, Lei Chenc, Ann Kuoc, Julie Lessard1, Alexey I. Nesvizhskiie, Jeff Ranishf, and Gerald R. Crabtreec, An embryonic stem cell chromatin remodelingcomplex, esBAF, is essential for embryonic stem cell self-renewal and pluripotency Proc Natl Acad Sci U S A. 2009 Mar 31;106(13):5181-6. Epub 2009 Mar 11. PMID: 19279220
Jiang Wu, Julie Lessard and Gerald R. Crabtree; Understanding the Words of Chromatin Regulation Cell 136:200-6, 2009 PMID: 19167321
Shih-Chu Kao, Hai Wu, Jianming Xie, Ching-Pin Chang, Jeffrey A. Ranish, Isabella A. Graef, and Gerald R. Crabtree; Calcineurin/NFAT Signaling Is Required for Neuregulin-Regulated Schwann Cell Differentiation Science 323:651-4, 2009 PMID: 19179536
Lazarevic V, Zullo AJ, Schweitzer MN, Staton TL, Gallo EM, Crabtree GR, Glimcher LH.The gene encoding early growth response 2, a target of the transcription factor NFAT, is required for the development and maturation of natural killer T cells. Nat Immunol. 2009 Mar;10(3):306-13. Epub 2009 Jan 25. PMID: 19169262
Elena M Gallo, Ho L, Winslow MM, Staton TL, Crabtree GR.Selective role of calcineurin in haematopoiesis and lymphopoiesis. EMBO Rep. 2008 Nov;9(11):1141-8. Epub 2008 Sep 26. PMID: 18818667
Gallo EM, Winslow MM, Canté-Barrett K, Radermacher AN, Ho L, McGinnis L, Iritani B, Neilson JR, Crabtree GR. Calcineurin sets the bandwidth for discrimination of signals during thymocyte development. Nature 2007 Nov 29;450(7170):731-5. PMID: 18046413
Lessard J, Wu JI, Ranish JA, Wan M, Winslow MM, Staahl BT, Wu H, Aebersold R, Graef IA, Crabtree GR .An essential switch in subunit composition of a chromatin remodeling complex during neural development. Neuron 55, 201-15 (2007). See comment by Rusty Gage Neuron. 2007 Jul 19;55(2):171-3.
Wu JI, Lessard J, Olave IA, Qiu Z, Ghosh A, Graef IA, Crabtree GR. Regulation of dendritic development by neuron-specific chromatin remodeling complexes Neuron 2007 Oct 4;56(1):94-108. PMID: 17920018
Liu, K. J., Arron, J. R., Stankunas, K., Crabtree, G. R. & Longaker, M. T. Chemical rescue of cleft palate and midline defects in conditional GSK-3beta mice. Nature 446, 79-82 (2007).
Heit, J. J. et al. Calcineurin/NFAT signalling regulates pancreatic beta-cell growth and function. Nature 443, 345-9 (2006).
Winslow MM, Gallo EM, Neilson JR, Crabtree GR.The calcineurin phosphatase complex modulates immunogenic B cell responses.
Immunity. 2006 Feb;24(2):141-52.PMID: 16473827
Arron, J. R. et al. NFAT dysregulation by increased dosage of DSCR1 and DYRK1A on chromosome 21. Nature 441, 595-600 (2006).
Gestwicki J, Crabtree GR and Graef IA Harnessing Chaperones to Generate Potent Small Molecule Inhibitors of Amyloid Aggregation. Science, Oct 29, 2004 p 865-869, PDF or HTML For information from the popular press see BBC News, or Scientific American (PDF), or CNN News (PDF), or Chemical Engineering News, or HHMI Report, or ABC News
Chang, CP, Neilson, J.R. Bayle, J.H., Gestwicki, J.E., Kuo, a. Stankunas, K. Graef, IA and Crabtree G.R. A Field of Calcineurin-NFAT signaling underlies heart valve morphogenesis, Cell Sept 3rd 2004. Note the cover design and the accompanying editorial. PDF or HTLM for the paper, PDF or HTML for the Editorial
Rose RB, Pullen KE, Bayle JH, Crabtree GR, Alber
T.Biochemical and structural basis for partially redundant
enzymatic and transcriptional functions of DCoH and DCoH2.
Biochemistry. 2004 Jun 15;43(23):7345-55
Parsons SA, Millay DP, Wilkins BJ, Bueno OF, Tsika
GL, Neilson JR, Liberatore CM, Yutzey KE, Crabtree GR, Tsika RW,
Molkentin JD. Genetic loss of calcineurin blocks mechanical overload-induced
skeletal muscle fiber type switching but not hypertrophy.
J Biol Chem. 2004 Jun 18;279(25):26192-200.
Brand M, Ranish JA, Kummer NT, Hamilton J, Igarashi
K, Francastel C, Chi TH, Crabtree GR, Aebersold R, Groudine M. Dynamic
changes in transcription factor complexes during erythroid differentiation
revealed by quantitative proteomics.
Nat Struct Mol Biol. 2004 Jan;11(1):73-80. PDF
Neilson JR, Winslow MM, Hur EM, Crabtree GR.
Calcineurin B1 is essential for positive but not negative selection
during thymocyte development. Immunity. 2004 Mar;20(3):255-66.
Stankunas et al. Condtional Protein Alleles Using Knock-in Mice and a Chemical Inducer of Dimerization Mol Cell Dec 12, 1615-1624, 2003.HTML PDF
Graef I.A. et al Neurotrophins and netrins require calcineurin/NFAT signaling to stimulate outgrowth of embryonic axons.Cell. 2003 May 30;113(5):657-70. HTML PDF
Chi et al. Sequential roles of Brg, the ATPase subunit of BAF chormatin remodeling complexes in thymocyte development. Immunity 2003, 169-182 HTML PDF
Chang CP, et al. Sonographic staging of the developmental status of mouse embryos in utero.Genesis. 2003 May;36(1):7-11. HTML
Olave IA, et al Nuclear actin and actin-related
proteins in chromatin remodeling.
Annu Rev Biochem. 2002;71:755-81. HTML
Crabtree GR, Olson EN. NFAT signaling: choreographing
the social lives of cells.
Cell. 2002 Apr;109 67-79. HTML PDF
Olave I, et al Identification of a polymorphic, neuron-specific chromatin remodeling complex.Genes Dev. 2002 Oct 1;16(19):2509-17. HTML PDF
Sheridan et al. Protein kinase A negatively modulates the nuclear accumulation of NF-ATc1 by priming for subsequent phosphorylation by glycogen synthase kinase-3.J Biol Chem. 2002 Dec 13;277(50):48664-76 HTML
Diehn M, et al .Genomic expression programs and the integration of the CD28 costimulatory signal in T cell activation.Proc Natl Acad Sci U S A. 2002 Sep 3;99(18):11796-801. HTML
Chi et al.Reciprocal regulation of CD4/CD8 expression by SWI/SNF-like BAF complexes.Nature. 2002 Jul 11;418(6894):195-9. HTML PDF
Bayle et. al. Hyperphenylalaninemia and impaired glucose tolerance in mice lacking the bifunctional DCoH gene.J Biol Chem. 2002 Aug 9;277(32):28884-91HTML
Rando OJ, et al. Phosphatidylinositol-dependent actin filament binding by the SWI/SNF-like BAF chromatin remodeling complex.Proc Natl Acad Sci U S A. 2002 Mar 5;99(5):2824-9. HTML
Graef IA, et al. Signals transduced by Ca(2+)/calcineurin and NFATc3/c4 pattern the developing vasculature.Cell. 2001 Jun 29;105(7):863-75 HTML PDF
Biggar SR, and Crabtree GR. Cell signaling can direct either binary or graded transcriptional responses.EMBO J. 2001 Jun 15;20(12):3167-76 HTML PDF
Graef IA, et al. Evolutionary relationships among Rel domains indicate functional diversification by recombination.Proc Natl Acad Sci U S A. 2001 May 8;98(10):5740-5 HTML
Graef IA, et al. L-type calcium channels and GSK-3 regulate the activity of NF-ATc4 in hippocampal neurons.Nature. 1999 Oct 14;401(6754):703-8 HTML PDF