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Located in rooms
B065 and B017
bklogo

 

DNA Sequencing
 

FREQUENTLY ASKED QUESTIONS
  1. Where are you located/contact info?
  2. What is your turnaround time?
  3. How much does it cost?
  4. What primers do you provide?
  5. What concentration should the DNA be?
  6. Why does my data look bad?
  7. Why can’t I download my data?



1. Where are you located/contact info?
We are located in the basement of the Beckman Center, B017.  We also have a drop-off area in cold room B024.

Phone: (650) 723-3189
Email: dnaseq@cmgm.stanford.edu
Shipping Address:        
DNA Sequencing/PAN Faciltiy
Beckman Center, B017
279 Campus Drive, West
Stanford, Ca 94305

2. What is your turnaround time?
When samples are dropped off by the deadline, results are linked on our website by Noon the following business day.

RXN’s NOT COMPLETED -  Drop-off deadline is 10:00am.
RXN’s COMLETED – Drop-off deadline is 2:00pm

3. How much does it cost?
Please check our current price list

4. What primers do you provide?
M13F  5’-TGTAAAACGACGGCCAGT-3’
M13R  5’- CAGGAAACAGCTATGACC-3’
T7        5’-TAATACGACTCACTATAGGG-3’
T3        5’-ATTAACCCTCACTAAACCCA-3’
SP6      5’-ATTTAGGTGACACTATAG-3’

5. What concentration should the DNA be?

Template

Concentration

Volume (for 2 rxns)

Double-stranded DNA

200ng/ul

10ul

PCR products

20ng/ul

10ul

Large Plasmids/BAC **

05-1.0ug

16ul

Primer

10pmol/ul

2ul

**For “Custom” primers, please add the 2ul of primer into your tubes, for a total volume of 12ul.

6. Why does my data look bad?

There are a few reasons for “bad” data.  Please go to our webpage “Data Interpretation” to find the cause and cure for bad data.  Remember most reruns and resequences are free.

7. Why can’t I download my data?
One reason maybe that there is a typo when creating the links, or the data has not finished downloading onto the server.  Please contact us immediately if you are unable retrieve your results.